標記核苷酸是生命科學研究的關(guān)鍵要素，是分子生物學中檢測特定核苷酸序列的有效工具，通過酶的作用即可結(jié)合到DNA/RNA序列中，進行測序和分析。由于出色的安全性、穩(wěn)定性和便捷性，生物素和熒光標記是用于原位雜交、基因表達譜分析或電泳遷移率轉(zhuǎn)移分析(EMSA)的核酸探針的最常見標記。

產(chǎn)品應用

核酸序列測定（Southern/Northern印記）

Hi-C技術(shù)，捕捉染色質(zhì)構(gòu)象

菌落與噬菌體，細胞與染色體的原位雜交

基因表達分析

治病病原體檢測

遺傳病與腫瘤診斷

品名：Aminoallyl-dUTP-XX-ATTO-532 Rhodamine-12-dUTP 

貨號：NU-803-RHOX

Aminoallyl-dUTP-XX-ATTO532 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Recommended Aminoallyl-dUTP-XX-ATTO532/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO532/ 50% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

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